quantification
The Accuris SmartDrop™ series of nano spectrophotometers provides quick and easy quantification of small samples. Utilizing a small sample volume eliminates the need for dilutions, providing more accurate results.
A full color, 7” touchscreen with icon-driven menus and preprogrammed protocols gets users up and running quickly. No external computer is required. Each unit features a quartz pedestal onto which a drop of sample is placed. The arm is lowered and the drop is stretched into a liquid column of specificed length for spectral analysis. DNA, RNA and protein concentrations and purity numbers are displayed in a matter of seconds.
The resulting data can be printed, saved in the SmartDrop’s onboard memory or exported via USB as an Excel file. All SmartDrop models include an integrated printer. For OD600 readings, each model includes a cuvette port and two optical glass cuvettes. Three SmartDrop models are available to meet a range of applications and budgets.
SmartDrop L is the affordable choice for laboratories needing to determine nucleic acid and protein concentrations and purity of samples. The LED light source and silicon photocell sensor provide readings at 260 and 280nm. Samples from 1.0 to 2.0µl are loaded directly on the pedestal. Results are provided in about 5 seconds. The software automatically
calculates and displays 260/280 ratios.
A full spectrum UV-Vis spectrophotometer, SmartDrop X requires 0.5 to 2.0µl of sample. In addition to nucleic acid and protein calculations, the software has preset programs for BCA, Bradford and Lowry assays. A full spectrum analysis can be performed in about 6 seconds, aiding in the
identification of unknowns. Up to 5 specific points on the spectrum can be selected for colorimetric readings output. The long life Xenon lamp source requires no warm up and is paired with a highly sensitive CCD detector. To provide the most accurate results, the SmartDrop X utilizes 2 path lengths.
This top of the line spectrophotometer can perform not only UV-Vis readings in the range of 200-800nm but also fluorescence readings. Like the SmartDrop X, the SmartDrop XF features predefined programs for nucleic acids, proteins, and various colorimetric assays. The patented, motorized system gently stretches the sample column and allows for three different path lengths, providing accurate readings of samples from 0.5 to 2.0µl without dilution.
The integrated fluorometer yields more accurate, sensitive and selective quantitation of nucleic acids. The blue light channel (460nm) is useful for detection of dyes such as PicoGreen and RiboGreen as well as GFP and fluorescein. UV, green and red channel models are available upon
request.
Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA, ssDNA and RNA using instruments such as the Qubit®, SmartDrop™ XF, and microplate readers. The reagents are highly selective for their target molecules; quantification results will not be skewed by alternative molecules in the samples. Included with each kit are a concentrated fluorescent assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, sample is added (1 µL to 20 µL), and the concentration determined using an appropriate fluorometer instrument that utilizes blue excitation (460 ± 20 nm) and measures green fluorescence (525-570 nm).
| Model | SmartDrop L (NS1000) | SmartDrop X (NS1010) | SmartDrop XF (NS1020) |
| Display | 7-inch touch screen | 7-inch touch screen | 7-inch touch screen |
| Minimum sample size | 1.0µl – 2.0µl | 0.5µl – 2.0µl | 0.5µl – 2.0µl |
| Path length | 0.5mm | 0.2mm, 1.0mm | 0.05mm, 0.2mm, 1.0mm |
| Light source | UV LED | Xenon Flash Lamp | Xenon Flash Lamp |
| Detector | UV Silicon Photocell | 2048 Element Linear Silicon CCD Array | 2048 Element Linear Silicon CCD Array |
| Wavelength | 260, 280, & 600nm | 200 – 800nm (1nm steps) | 200 – 800nm (1nm steps) |
| Absorbance range | 0.2 – 50 A (10mm equivalent) | 0.04 – 90 A (10mm equivalent) | 0.04 – 300 A (10mm equivalent) |
| Absorbance precision | 0.005 A | 0.003 A | 0.003 A |
| Lower limit of detection | dsDNA: 10ng/µl; BSA: 0.1 mg/ml | dsDNA: 2ng/µl; BSA: 0.1mg/ml | dsDNA: 2ng/µl ; BSA: 0.1mg/ml |
| Upper limit of detection | dsDNA: 2,500ng/µl; BSA: 75.0 mg/ml | dsDNA: 4,500ng/µl; BSA: 135mg/ml | dsDNA: 15,000ng/µl; BSA: 360mg/ml |
| Detection time | < 6 seconds | < 5 seconds | < 6 seconds |
| Communication ports | USB for data export | USB for data export | USB for data export |
| Voltage input | DC 24V, 2A | DC 24V, 2A | DC 24V, 2A |
| Power | 25W | 25W | 25W |
| Dimensions (WxDxH) | 8.2 x 11.0 x 7.3 in. / 20.8 x 28.0 x 18.6cm | 8.3 x 10.6 x 7.2 in. / 21.0 x 26.8 x 18.1cm | 8.2 x 12.6 x 7.3 in. / 20.8 x 32.0 x 18.6cm |
| Weight | 3.6 kg / 7.9 lbs | 2.8 kg / 6.2 lbs | 3.6 kg / 7.9 lbs |
| Cuvette OD600 detection | |||
| Absorbance range | 0.0 – 4.0 A | 0.0 – 4.0 A | 0.0 – 4.0 A |
| Absorbance stability | [0,3) ≤ 0.5%, [3,4) ≤ 2% | [0,3) ≤ 0.5%, [3,4) ≤ 2% | [0,3) ≤ 0.5%, [3,4) ≤ 2% |
| Absorbance repeatibility | [0,3) ≤ 0.5%, [3,4) ≤ 2% | [0,3) ≤ 0.5%, [3,4) ≤ 2% | [0,3) ≤ 0.5%, [3,4) ≤ 2% |
| Absorbance precision | [0,2) ≤ 0.005 A, [2,3) ≤ 1%, [3,4) ≤ 2% | [0,2) ≤ 0.005 A, [2,3) ≤ 1%, [3,4) ≤ 2% | [0,2) ≤ 0.005 A, [2,3) ≤ 1%, [3,4) ≤ 2% |
| Fluorescence detection | |||
| Excitation/Emission | N/A | N/A | 460 ± 20nm / 525-570nm |
| Sensitivity | N/A | N/A | dsDNA: 0.5pg/µl |
| Linearity | N/A | N/A | R² ≥ 0.995 |
| Repeatability | N/A | N/A | ≤ 1.5% |
| Stability | N/A | N/A | ≤ 1.5% |
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